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Image Search Results


Journal: MethodsX

Article Title: FACS-Free isolation and purification protocol of mouse prostate epithelial cells for organoid primary culture

doi: 10.1016/j.mex.2022.101843

Figure Lengend Snippet:

Article Snippet: Reagents/tools: , • HBSS 1X (Wisent, 311-513-CL) • HEPES 100 mM (Wisent, 330-050-EL) • Penicillin-streptomycin solution (Wisent, 450-200-EL) • EpiCult B Medium Mouse Kit (Stemcell Technologies, 05610) • Human recombinant EGF (Stemcell Technologies, 78006) • Human recombinant bFGF (Stemcell Technologies, 78134) • Heparin sodium salt from porcine intestinal mucosa (Sigma Aldrich, H3393) • Gentle collagenase/hyaluronidase (StemCell Technologies, 07919) • Corning® Matrigel® Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-free, LDEV-free (Corning, 356231) • 0.25% trypsin-EDTA (Wisent, 325-043-EL) • Dispase 5U/mL (Stemcell Technologies, 07913) • DNase I (Sigma Aldrich, 11284932001) • Falcon 40 μm Cell Strainer (Fisher Scientific, 08-771-1) • EasySep Mouse PE Positive Selection Kit II (StemCell Technologies, 17666) • PE Rat Anti-Human CD49f (BD Biosciences, 555736).

Techniques: Isolation, Purification, Recombinant, Gentle, Membrane, Selection, Magnetic Beads, Ex Vivo, Flow Cytometry, In Vivo

Journal: MethodsX

Article Title: FACS-Free isolation and purification protocol of mouse prostate epithelial cells for organoid primary culture

doi: 10.1016/j.mex.2022.101843

Figure Lengend Snippet:

Article Snippet: Reagents/tools: , • HBSS 1X (Wisent, 311-513-CL) • HEPES 100 mM (Wisent, 330-050-EL) • Penicillin-streptomycin solution (Wisent, 450-200-EL) • EpiCult B Medium Mouse Kit (Stemcell Technologies, 05610) • Human recombinant EGF (Stemcell Technologies, 78006) • Human recombinant bFGF (Stemcell Technologies, 78134) • Heparin sodium salt from porcine intestinal mucosa (Sigma Aldrich, H3393) • Gentle collagenase/hyaluronidase (StemCell Technologies, 07919) • Corning® Matrigel® Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-free, LDEV-free (Corning, 356231) • 0.25% trypsin-EDTA (Wisent, 325-043-EL) • Dispase 5U/mL (Stemcell Technologies, 07913) • DNase I (Sigma Aldrich, 11284932001) • Falcon 40 μm Cell Strainer (Fisher Scientific, 08-771-1) • EasySep Mouse PE Positive Selection Kit II (StemCell Technologies, 17666) • PE Rat Anti-Human CD49f (BD Biosciences, 555736).

Techniques: Isolation, Purification, Recombinant, Gentle, Membrane, Selection, Magnetic Beads, Ex Vivo, Flow Cytometry, In Vivo

Journal: MethodsX

Article Title: FACS-Free isolation and purification protocol of mouse prostate epithelial cells for organoid primary culture

doi: 10.1016/j.mex.2022.101843

Figure Lengend Snippet:

Article Snippet: Reagents/tools: , • HBSS 1X (Wisent, 311-513-CL) • HEPES 100 mM (Wisent, 330-050-EL) • Penicillin-streptomycin solution (Wisent, 450-200-EL) • EpiCult B Medium Mouse Kit (Stemcell Technologies, 05610) • Human recombinant EGF (Stemcell Technologies, 78006) • Human recombinant bFGF (Stemcell Technologies, 78134) • Heparin sodium salt from porcine intestinal mucosa (Sigma Aldrich, H3393) • Gentle collagenase/hyaluronidase (StemCell Technologies, 07919) • Corning® Matrigel® Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-free, LDEV-free (Corning, 356231) • 0.25% trypsin-EDTA (Wisent, 325-043-EL) • Dispase 5U/mL (Stemcell Technologies, 07913) • DNase I (Sigma Aldrich, 11284932001) • Falcon 40 μm Cell Strainer (Fisher Scientific, 08-771-1) • EasySep Mouse PE Positive Selection Kit II (StemCell Technologies, 17666) • PE Rat Anti-Human CD49f (BD Biosciences, 555736).

Techniques: Isolation, Purification, Recombinant, Gentle, Membrane, Selection, Magnetic Beads, Ex Vivo, Flow Cytometry, In Vivo

Journal: MethodsX

Article Title: FACS-Free isolation and purification protocol of mouse prostate epithelial cells for organoid primary culture

doi: 10.1016/j.mex.2022.101843

Figure Lengend Snippet:

Article Snippet: Reagents/tools: , • HBSS 1X (Wisent, 311-513-CL) • HEPES 100 mM (Wisent, 330-050-EL) • Penicillin-streptomycin solution (Wisent, 450-200-EL) • EpiCult B Medium Mouse Kit (Stemcell Technologies, 05610) • Human recombinant EGF (Stemcell Technologies, 78006) • Human recombinant bFGF (Stemcell Technologies, 78134) • Heparin sodium salt from porcine intestinal mucosa (Sigma Aldrich, H3393) • Gentle collagenase/hyaluronidase (StemCell Technologies, 07919) • Corning® Matrigel® Growth Factor Reduced (GFR) Basement Membrane Matrix, Phenol Red-free, LDEV-free (Corning, 356231) • 0.25% trypsin-EDTA (Wisent, 325-043-EL) • Dispase 5U/mL (Stemcell Technologies, 07913) • DNase I (Sigma Aldrich, 11284932001) • Falcon 40 μm Cell Strainer (Fisher Scientific, 08-771-1) • EasySep Mouse PE Positive Selection Kit II (StemCell Technologies, 17666) • PE Rat Anti-Human CD49f (BD Biosciences, 555736).

Techniques: Isolation, Purification, Recombinant, Gentle, Membrane, Selection, Magnetic Beads, Ex Vivo, Flow Cytometry, In Vivo